Acetaminophen toxicity EMCrit Project Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen, also known as Tylenol, is used to help with pain & to reduce fever. However, taking too much acetaminophen can be harmful to the liver which filters out toxins & g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram