Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Frontiers Liquid Chromatography Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S Transferases Could Tylenol be making your child's health worse? Acetaminophen is widely used for pain and fever relief, but research suggests it depletes glutathione, a powerful antioxidant essential for immune function,