Cysteine conjugates of acetaminophen and p aminophenol are potent inducers of cellular impairment in human proximal tubular kidney HK 2 cells Archives of Toxicology Springer Nature Link Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism of acetaminophen and Old and New Analgesic Acetaminophen: Pharmacological Mechanisms Compared with Non Steroidal Anti Inflammatory Drugs Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.