Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Painkillers and the Microbiome: The Hidden Cost to Gut Health Traditional & Natural Health Alliance (TNHA) Drug Induced Nutrition Depletion Replenish Your System Traditional & Natural Health Alliance (TNHA) Ibuprofen administration attenuates serum TNF levels, hepatic glutathione depletion, hepatic apoptosis and mouse mortality after Fas stimulation ScienceDirect