Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione: The Master Antioxidant and Its Remarkable Benefits Medical Aesthetics & Medical Spa located in West Hollywood, CA me.losangeles aesthetics Glutathione Deficiency Symptoms: Is Poor Sleep Just the Beginning? Cardiology & Neurology Specialists located in Queens, Forest Hills and Brighton Beach, Brooklyn, NY Advanced Medical Care Can Tylenol (acetaminophen) deplete glutathione levels in the body?