Biochemistry Metabolism of acetaminophen (paracetamol) . Acetaminophen is widely used as a painkiller. Taken in the usual therapeutic doses, it is Instagram Acetaminophen Induced Hepatotoxicity in Obesity and Nonalcoholic Fatty Liver Disease: A Critical Review Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity