Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols Sepharose 4B GE Life Sciences 17 0120 01, pack 1L 9012 36 6 Glutathione Sepharose 4B GST tagged protein purification resin Cytiva Glutathione Sepharose 4B, Cytiva, affinity resin, pack of 10 mL
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