Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Tylenol lowers glutathione, which is why it can cause liver failure. Low glutathione can cause many serious health problems that can vary from person to person. Finding out if you have genes that can Tylenol's Impact on Glutathione and Liver Damage