Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol Avoid or Not to Avoid? Acetaminophen (Tylenol) can deplete glutathione, your body's most important detox antioxidant. This matters even more if your child has an MTHFR gene mutation, which I've posted this every year for 7 years. PSA please share. The horror stories from Tylenol (Acetaminophen) usage for children are hard to digest literally. The way my mind The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram