Transglutaminase 2 crosslinks the glutathione S transferase tag, impeding proteinprotein interactions of the fused protein Experimental & Molecular Medicine On and Off Column Protein Affinity Tag Removal Strategic optimization of conditions for the solubilization of GST tagged amphipathic helix containing ciliary proteins overexpressed as inclusion bodies in E. coli Microbial Cell Factories Springer Nature Link Quantitative GST pull down for binding affinity determination. Download Scientific Diagram
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gst glutathione binding affinity Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins