Glutamine and Cancer: How It Works, What to Do Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen, Acetylcysteine, and Anaphylaxis With a Twist BROWN EMERGENCY MEDICINE BLOG